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    哈靈文獻:指標C57BL/6J 小鼠腭突中嵴上皮細胞的原代細胞

       熱烈祝賀 重慶醫科大學 陳強老師  文獻發表成功

     

    文獻主題:

    腭部間充質干細胞生物學行為的比較

    2,3,7,8-四氯二苯并對二惡英體外誘導的上皮細胞

     

    以下是相關檢測指標

    dical University Animal Center were used in this study. They were
    housed under constant conditions at a temperature of 22 ± 2 °C, hu
    midity of 50 ± 10 %, and 12 -h light/dark cycle. Female mice were
    crossed with males in the evening and checked for vaginal plugs the
    next morning. Additionally, their weights were recorded (embryonic
    day 0, E0). On gestation day 14 (GD14), pregnant mice with weight
    gain greater than 2 g were sacrifificed for primary mesenchymal cells. All
    experiments abided by the Institutional Ethical Guidelines for
    Experiments with Animals.
    2.2. Cells and medium
    Epithelial cells and medium were obtained from Shanghai Haling
    Biotechnology Company, Limited. Primary epithelial cells were derived
    from the palatal tissue of GD14 embryos from C57BL/6 mice.
    Immunoflfluorescence was performed to detect the expression of the
    epithelial marker ZO-1, and microscopic morphology was used to
    confifirm its shape. A specifific medium was used to grow the epithelial
    cells.
    Mesenchymal cells were obtained from the palatal tissue of GD14
    embryos from C57BL/6 mice. The methods of mesenchymal cell ac
    quisition and culture were according to the previous study (Feng et al.,
    2013). Immunoflfluorescence was performed to detect the expression of
    the mesenchymal marker vimentin, and microscopic morphology was
    used to confifirm its form. The media were DMEM/F12 (1:1) basic (1×)
    (Gibco, USA) with 10 % fetal calf serum (FBS, Sijiqing, China).

     

    文獻鏈接:

    鏈接: https://pan.baidu.com/s/1APCNbuak_Q5CiruhkW1HKg 提取碼: bdg9

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