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    哈靈文獻:miR-4504參與三角帆蚌珍珠層顏色的形成

    熱烈祝賀 上海海洋大學 -陳夏君 陳老師 文獻發表成功

    文獻主題:

    miR-4504參與三角帆蚌珍珠層顏色的形成

    文獻中引用的我司產品(加亮部分):

    Mantle samples (500mg middle mantle and 500mg fringe

    mantle from each mussel) were collected from groups A, N and P.

    Samples were immediately frozen in liquid nitrogen and stored

    at 80 C until use. Tyrosinase activity was measured using the

    Tissue Tyrosinase Activity Assay Kit (HaLing, Shanghai, China), according

    to the manufacturer's instructions. Mantle samples were

    comminuted in liquid nitrogen and treated with lysis buffer. After

    centrifugation, the supernatant was collected and mixed with

    buffer and tyrosine. All samples were incubated at 25 C for 1 h.

    Tyrosinase activity was measured using a microplate reader with

    the dopachrome content at 475 nm (UV-3010; Shimadzu, Japan).

    One-way ANOVAs were performed using SPSS 17.0 to determine

    significant differences between groups. Data from the tyrosinase

    activity experiments were expressed as the mean ± SD (n ¼ 6). A pvalue

    of <0.05 was considered statistically significant.

    文獻鏈接:

    鏈接: https://pan.baidu.com/s/1XoZQb0hjYlsTIV4aE1Zt-Q 提取碼: ps5k

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